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abc immunoperoxidase staining avidin biotin complexes  (Vector Laboratories)


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    Vector Laboratories abc immunoperoxidase staining avidin biotin complexes
    Examples of optimal and suboptimal concordance with PD-L1 IHC reference stains for different protocols during the 2018 EQA scheme. Images represent a matching core with a validated consensus TPS of > 50%. The core was part of a tissue micro-array containing four different FFPE cases for staining by routine antibodies and detection systems of the 2018 EQA participants. Protocols are presented as reported by the participating laboratories. Scale bar 1 mm. Optimal concordance (top row, from left to right): SP263: RTU antibody from Ventana (16 min incubation at 36 °C) in combination with the Ventana CC1 (64 min.) and OptiView DAB IHC Detection Kit. 22C3: Antibody from Dako (diluted 1/40, 16-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. 28-8: Antibody from Abcam (diluted 1/100, 32-min incubation at RT) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. SP142: RTU antibody from Ventana (24-min incubation at 37 °C) in combination with the Ventana CC1 (48 min) and UltraView DAB IHC Detection Kit. E1L3N: Antibody from Cell Signaling (diluted 1/200, 30-min incubation at RT) in combination with Leica Bond Epitope Retrieval 2 (20 min) and bond polymer refine detection system. Suboptimal concordance (bottom row, from left to right): SP263: RTU antibody from Ventana (60-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit; weak demonstration of antigen in the tumor population and cytoplasmic staining. 22C3: Antibody from Dako (diluted 1/50, 30-min incubation at RT) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; Excessive background staining and cytoplasmic staining. 28-8: Antibody from Abcam (diluted 1/50, 20-min incubation at 32 °C) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; background staining. SP142: RTU antibody from Ventana (16-min incubation at 36 °C) in combination with the Ventana CC1 (48 min) and OptiView DAB IHC Detection Kit; weak staining of epithelial cells. E1L3N: Antibody from Cell Signaling (diluted 1/150, 60-min incubation at RT) in combination with laboratory developed antigen retrieval by TRIS-EDTA and the Vectastain <t>ABC</t> <t>immunoperoxidase</t> staining avidin-biotin complexes; weak demonstration of antigen in the tumor population and cytoplasmic staining. Abbreviations: EQA external quality assessment, FFPE formalin-fixed paraffin embedded, IHC immunohistochemistry, PD-L1 programmed death ligand 1, RT room temperature, RTU ready-to-use, TPS tumor proportion score
    Abc Immunoperoxidase Staining Avidin Biotin Complexes, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abc immunoperoxidase staining avidin biotin complexes/product/Vector Laboratories
    Average 96 stars, based on 2245 article reviews
    abc immunoperoxidase staining avidin biotin complexes - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "PD-L1 immunohistochemistry in non-small-cell lung cancer: unraveling differences in staining concordance and interpretation"

    Article Title: PD-L1 immunohistochemistry in non-small-cell lung cancer: unraveling differences in staining concordance and interpretation

    Journal: Virchows Archiv

    doi: 10.1007/s00428-020-02976-5

    Examples of optimal and suboptimal concordance with PD-L1 IHC reference stains for different protocols during the 2018 EQA scheme. Images represent a matching core with a validated consensus TPS of > 50%. The core was part of a tissue micro-array containing four different FFPE cases for staining by routine antibodies and detection systems of the 2018 EQA participants. Protocols are presented as reported by the participating laboratories. Scale bar 1 mm. Optimal concordance (top row, from left to right): SP263: RTU antibody from Ventana (16 min incubation at 36 °C) in combination with the Ventana CC1 (64 min.) and OptiView DAB IHC Detection Kit. 22C3: Antibody from Dako (diluted 1/40, 16-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. 28-8: Antibody from Abcam (diluted 1/100, 32-min incubation at RT) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. SP142: RTU antibody from Ventana (24-min incubation at 37 °C) in combination with the Ventana CC1 (48 min) and UltraView DAB IHC Detection Kit. E1L3N: Antibody from Cell Signaling (diluted 1/200, 30-min incubation at RT) in combination with Leica Bond Epitope Retrieval 2 (20 min) and bond polymer refine detection system. Suboptimal concordance (bottom row, from left to right): SP263: RTU antibody from Ventana (60-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit; weak demonstration of antigen in the tumor population and cytoplasmic staining. 22C3: Antibody from Dako (diluted 1/50, 30-min incubation at RT) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; Excessive background staining and cytoplasmic staining. 28-8: Antibody from Abcam (diluted 1/50, 20-min incubation at 32 °C) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; background staining. SP142: RTU antibody from Ventana (16-min incubation at 36 °C) in combination with the Ventana CC1 (48 min) and OptiView DAB IHC Detection Kit; weak staining of epithelial cells. E1L3N: Antibody from Cell Signaling (diluted 1/150, 60-min incubation at RT) in combination with laboratory developed antigen retrieval by TRIS-EDTA and the Vectastain ABC immunoperoxidase staining avidin-biotin complexes; weak demonstration of antigen in the tumor population and cytoplasmic staining. Abbreviations: EQA external quality assessment, FFPE formalin-fixed paraffin embedded, IHC immunohistochemistry, PD-L1 programmed death ligand 1, RT room temperature, RTU ready-to-use, TPS tumor proportion score
    Figure Legend Snippet: Examples of optimal and suboptimal concordance with PD-L1 IHC reference stains for different protocols during the 2018 EQA scheme. Images represent a matching core with a validated consensus TPS of > 50%. The core was part of a tissue micro-array containing four different FFPE cases for staining by routine antibodies and detection systems of the 2018 EQA participants. Protocols are presented as reported by the participating laboratories. Scale bar 1 mm. Optimal concordance (top row, from left to right): SP263: RTU antibody from Ventana (16 min incubation at 36 °C) in combination with the Ventana CC1 (64 min.) and OptiView DAB IHC Detection Kit. 22C3: Antibody from Dako (diluted 1/40, 16-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. 28-8: Antibody from Abcam (diluted 1/100, 32-min incubation at RT) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. SP142: RTU antibody from Ventana (24-min incubation at 37 °C) in combination with the Ventana CC1 (48 min) and UltraView DAB IHC Detection Kit. E1L3N: Antibody from Cell Signaling (diluted 1/200, 30-min incubation at RT) in combination with Leica Bond Epitope Retrieval 2 (20 min) and bond polymer refine detection system. Suboptimal concordance (bottom row, from left to right): SP263: RTU antibody from Ventana (60-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit; weak demonstration of antigen in the tumor population and cytoplasmic staining. 22C3: Antibody from Dako (diluted 1/50, 30-min incubation at RT) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; Excessive background staining and cytoplasmic staining. 28-8: Antibody from Abcam (diluted 1/50, 20-min incubation at 32 °C) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; background staining. SP142: RTU antibody from Ventana (16-min incubation at 36 °C) in combination with the Ventana CC1 (48 min) and OptiView DAB IHC Detection Kit; weak staining of epithelial cells. E1L3N: Antibody from Cell Signaling (diluted 1/150, 60-min incubation at RT) in combination with laboratory developed antigen retrieval by TRIS-EDTA and the Vectastain ABC immunoperoxidase staining avidin-biotin complexes; weak demonstration of antigen in the tumor population and cytoplasmic staining. Abbreviations: EQA external quality assessment, FFPE formalin-fixed paraffin embedded, IHC immunohistochemistry, PD-L1 programmed death ligand 1, RT room temperature, RTU ready-to-use, TPS tumor proportion score

    Techniques Used: Microarray, Staining, Incubation, Immunoperoxidase Staining, Avidin-Biotin Assay, Formalin-fixed Paraffin-Embedded, Immunohistochemistry

    Analysis failures, TPS misclassifications, and ESS for the different PD-L1 IHC protocols used in the EQA schemes
    Figure Legend Snippet: Analysis failures, TPS misclassifications, and ESS for the different PD-L1 IHC protocols used in the EQA schemes

    Techniques Used: Immunoperoxidase Staining, Avidin-Biotin Assay, Plasmid Preparation



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    Examples of optimal and suboptimal concordance with PD-L1 IHC reference stains for different protocols during the 2018 EQA scheme. Images represent a matching core with a validated consensus TPS of > 50%. The core was part of a tissue micro-array containing four different FFPE cases for staining by routine antibodies and detection systems of the 2018 EQA participants. Protocols are presented as reported by the participating laboratories. Scale bar 1 mm. Optimal concordance (top row, from left to right): SP263: RTU antibody from Ventana (16 min incubation at 36 °C) in combination with the Ventana CC1 (64 min.) and OptiView DAB IHC Detection Kit. 22C3: Antibody from Dako (diluted 1/40, 16-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. 28-8: Antibody from Abcam (diluted 1/100, 32-min incubation at RT) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. SP142: RTU antibody from Ventana (24-min incubation at 37 °C) in combination with the Ventana CC1 (48 min) and UltraView DAB IHC Detection Kit. E1L3N: Antibody from Cell Signaling (diluted 1/200, 30-min incubation at RT) in combination with Leica Bond Epitope Retrieval 2 (20 min) and bond polymer refine detection system. Suboptimal concordance (bottom row, from left to right): SP263: RTU antibody from Ventana (60-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit; weak demonstration of antigen in the tumor population and cytoplasmic staining. 22C3: Antibody from Dako (diluted 1/50, 30-min incubation at RT) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; Excessive background staining and cytoplasmic staining. 28-8: Antibody from Abcam (diluted 1/50, 20-min incubation at 32 °C) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; background staining. SP142: RTU antibody from Ventana (16-min incubation at 36 °C) in combination with the Ventana CC1 (48 min) and OptiView DAB IHC Detection Kit; weak staining of epithelial cells. E1L3N: Antibody from Cell Signaling (diluted 1/150, 60-min incubation at RT) in combination with laboratory developed antigen retrieval by TRIS-EDTA and the Vectastain <t>ABC</t> <t>immunoperoxidase</t> staining avidin-biotin complexes; weak demonstration of antigen in the tumor population and cytoplasmic staining. Abbreviations: EQA external quality assessment, FFPE formalin-fixed paraffin embedded, IHC immunohistochemistry, PD-L1 programmed death ligand 1, RT room temperature, RTU ready-to-use, TPS tumor proportion score
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    Examples of optimal and suboptimal concordance with PD-L1 IHC reference stains for different protocols during the 2018 EQA scheme. Images represent a matching core with a validated consensus TPS of > 50%. The core was part of a tissue micro-array containing four different FFPE cases for staining by routine antibodies and detection systems of the 2018 EQA participants. Protocols are presented as reported by the participating laboratories. Scale bar 1 mm. Optimal concordance (top row, from left to right): SP263: RTU antibody from Ventana (16 min incubation at 36 °C) in combination with the Ventana CC1 (64 min.) and OptiView DAB IHC Detection Kit. 22C3: Antibody from Dako (diluted 1/40, 16-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. 28-8: Antibody from Abcam (diluted 1/100, 32-min incubation at RT) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. SP142: RTU antibody from Ventana (24-min incubation at 37 °C) in combination with the Ventana CC1 (48 min) and UltraView DAB IHC Detection Kit. E1L3N: Antibody from Cell Signaling (diluted 1/200, 30-min incubation at RT) in combination with Leica Bond Epitope Retrieval 2 (20 min) and bond polymer refine detection system. Suboptimal concordance (bottom row, from left to right): SP263: RTU antibody from Ventana (60-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit; weak demonstration of antigen in the tumor population and cytoplasmic staining. 22C3: Antibody from Dako (diluted 1/50, 30-min incubation at RT) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; Excessive background staining and cytoplasmic staining. 28-8: Antibody from Abcam (diluted 1/50, 20-min incubation at 32 °C) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; background staining. SP142: RTU antibody from Ventana (16-min incubation at 36 °C) in combination with the Ventana CC1 (48 min) and OptiView DAB IHC Detection Kit; weak staining of epithelial cells. E1L3N: Antibody from Cell Signaling (diluted 1/150, 60-min incubation at RT) in combination with laboratory developed antigen retrieval by TRIS-EDTA and the Vectastain <t>ABC</t> <t>immunoperoxidase</t> staining avidin-biotin complexes; weak demonstration of antigen in the tumor population and cytoplasmic staining. Abbreviations: EQA external quality assessment, FFPE formalin-fixed paraffin embedded, IHC immunohistochemistry, PD-L1 programmed death ligand 1, RT room temperature, RTU ready-to-use, TPS tumor proportion score
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    Image Search Results


    Examples of optimal and suboptimal concordance with PD-L1 IHC reference stains for different protocols during the 2018 EQA scheme. Images represent a matching core with a validated consensus TPS of > 50%. The core was part of a tissue micro-array containing four different FFPE cases for staining by routine antibodies and detection systems of the 2018 EQA participants. Protocols are presented as reported by the participating laboratories. Scale bar 1 mm. Optimal concordance (top row, from left to right): SP263: RTU antibody from Ventana (16 min incubation at 36 °C) in combination with the Ventana CC1 (64 min.) and OptiView DAB IHC Detection Kit. 22C3: Antibody from Dako (diluted 1/40, 16-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. 28-8: Antibody from Abcam (diluted 1/100, 32-min incubation at RT) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. SP142: RTU antibody from Ventana (24-min incubation at 37 °C) in combination with the Ventana CC1 (48 min) and UltraView DAB IHC Detection Kit. E1L3N: Antibody from Cell Signaling (diluted 1/200, 30-min incubation at RT) in combination with Leica Bond Epitope Retrieval 2 (20 min) and bond polymer refine detection system. Suboptimal concordance (bottom row, from left to right): SP263: RTU antibody from Ventana (60-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit; weak demonstration of antigen in the tumor population and cytoplasmic staining. 22C3: Antibody from Dako (diluted 1/50, 30-min incubation at RT) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; Excessive background staining and cytoplasmic staining. 28-8: Antibody from Abcam (diluted 1/50, 20-min incubation at 32 °C) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; background staining. SP142: RTU antibody from Ventana (16-min incubation at 36 °C) in combination with the Ventana CC1 (48 min) and OptiView DAB IHC Detection Kit; weak staining of epithelial cells. E1L3N: Antibody from Cell Signaling (diluted 1/150, 60-min incubation at RT) in combination with laboratory developed antigen retrieval by TRIS-EDTA and the Vectastain ABC immunoperoxidase staining avidin-biotin complexes; weak demonstration of antigen in the tumor population and cytoplasmic staining. Abbreviations: EQA external quality assessment, FFPE formalin-fixed paraffin embedded, IHC immunohistochemistry, PD-L1 programmed death ligand 1, RT room temperature, RTU ready-to-use, TPS tumor proportion score

    Journal: Virchows Archiv

    Article Title: PD-L1 immunohistochemistry in non-small-cell lung cancer: unraveling differences in staining concordance and interpretation

    doi: 10.1007/s00428-020-02976-5

    Figure Lengend Snippet: Examples of optimal and suboptimal concordance with PD-L1 IHC reference stains for different protocols during the 2018 EQA scheme. Images represent a matching core with a validated consensus TPS of > 50%. The core was part of a tissue micro-array containing four different FFPE cases for staining by routine antibodies and detection systems of the 2018 EQA participants. Protocols are presented as reported by the participating laboratories. Scale bar 1 mm. Optimal concordance (top row, from left to right): SP263: RTU antibody from Ventana (16 min incubation at 36 °C) in combination with the Ventana CC1 (64 min.) and OptiView DAB IHC Detection Kit. 22C3: Antibody from Dako (diluted 1/40, 16-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. 28-8: Antibody from Abcam (diluted 1/100, 32-min incubation at RT) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. SP142: RTU antibody from Ventana (24-min incubation at 37 °C) in combination with the Ventana CC1 (48 min) and UltraView DAB IHC Detection Kit. E1L3N: Antibody from Cell Signaling (diluted 1/200, 30-min incubation at RT) in combination with Leica Bond Epitope Retrieval 2 (20 min) and bond polymer refine detection system. Suboptimal concordance (bottom row, from left to right): SP263: RTU antibody from Ventana (60-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit; weak demonstration of antigen in the tumor population and cytoplasmic staining. 22C3: Antibody from Dako (diluted 1/50, 30-min incubation at RT) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; Excessive background staining and cytoplasmic staining. 28-8: Antibody from Abcam (diluted 1/50, 20-min incubation at 32 °C) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; background staining. SP142: RTU antibody from Ventana (16-min incubation at 36 °C) in combination with the Ventana CC1 (48 min) and OptiView DAB IHC Detection Kit; weak staining of epithelial cells. E1L3N: Antibody from Cell Signaling (diluted 1/150, 60-min incubation at RT) in combination with laboratory developed antigen retrieval by TRIS-EDTA and the Vectastain ABC immunoperoxidase staining avidin-biotin complexes; weak demonstration of antigen in the tumor population and cytoplasmic staining. Abbreviations: EQA external quality assessment, FFPE formalin-fixed paraffin embedded, IHC immunohistochemistry, PD-L1 programmed death ligand 1, RT room temperature, RTU ready-to-use, TPS tumor proportion score

    Article Snippet: , Homebrew EDTA or TRIS-EDTA (w/wo pressure cooker) , ABC immunoperoxidase staining avidin-biotin complexes (Vectastain ABC Elite; Vector Laboratories) , 1 (0.7%) , 0 (0.0%) , 1 (5.0%) , 0 (0.0%) , 1.0.

    Techniques: Microarray, Staining, Incubation, Immunoperoxidase Staining, Avidin-Biotin Assay, Formalin-fixed Paraffin-Embedded, Immunohistochemistry

    Analysis failures, TPS misclassifications, and ESS for the different PD-L1 IHC protocols used in the EQA schemes

    Journal: Virchows Archiv

    Article Title: PD-L1 immunohistochemistry in non-small-cell lung cancer: unraveling differences in staining concordance and interpretation

    doi: 10.1007/s00428-020-02976-5

    Figure Lengend Snippet: Analysis failures, TPS misclassifications, and ESS for the different PD-L1 IHC protocols used in the EQA schemes

    Article Snippet: , Homebrew EDTA or TRIS-EDTA (w/wo pressure cooker) , ABC immunoperoxidase staining avidin-biotin complexes (Vectastain ABC Elite; Vector Laboratories) , 1 (0.7%) , 0 (0.0%) , 1 (5.0%) , 0 (0.0%) , 1.0.

    Techniques: Immunoperoxidase Staining, Avidin-Biotin Assay, Plasmid Preparation